› 2wvs

X-ray diffraction
2.19Å resolution

Crystal structure of an alpha-L-fucosidase GH29 trapped covalent intermediate from Bacteroides thetaiotaomicron in complex with 2- fluoro-fucosyl fluoride using an E288Q mutant

Released:
DOI: 10.2210/pdb2wvs/pdb
PDB entry 2wvs coloured by chain, front view.
PDB entry 2wvs coloured by chain, side view.
PDB entry 2wvs coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
Analysis of the reaction coordinate of alpha-L-fucosidases: a combined structural and quantum mechanical approach.
Lammerts van Bueren A, Ardèvol A, Fayers-Kerr J, Luo B, Zhang Y, Sollogoub M, Blériot Y, Rovira C, Davies GJ
J Am Chem Soc 132 1804-6 (2010)
PMID: 20092273

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183665 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-L-fucosidase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 443 amino acids
Theoretical weight: 51.33 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8A3I4 (Residues: 31-473; Coverage: 97%)
Gene name: BT_2970
Sequence domains: Alpha-L-fucosidase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FUF 5 x FUF
Ligand SO4 7 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P21
Unit cell:
a: 54.84Å b: 185.16Å c: 97.87Å
α: 90° β: 94.57° γ: 90°
R-values:
R R work R free
0.181 0.178 0.235
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Mucin glycan foraging in the human gut microbiome.
Tailford et al. (2015)
4 more

5 mentions without citation

Therapeutic strategies for anchored kinases and phosphatases: exploiting short linear motifs and intrinsic disorder.
Nygren et al. (2015)
4 more