› 2ww3

X-ray diffraction
2.1Å resolution

Structure of the Family GH92 Inverting Mannosidase BT3990 from Bacteroides thetaiotaomicron VPI-5482 in complex with thiomannobioside

Released:
DOI: 10.2210/pdb2ww3/pdb
PDB entry 2ww3 coloured by chain, front view.
PDB entry 2ww3 coloured by chain, side view.
PDB entry 2ww3 coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
Mechanistic insights into a Ca2+-dependent family of alpha-mannosidases in a human gut symbiont.
Zhu Y, Suits MD, Thompson AJ, Chavan S, Dinev Z, Dumon C, Smith N, Moremen KW, Xiang Y, Siriwardena A, Williams SJ, Gilbert HJ, Davies GJ
Nat Chem Biol 6 125-32 (2010)
PMID: 20081828

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183595 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Alpha-1,2-mannosidase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 744 amino acids
Theoretical weight: 85.69 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8A0N1 (Residues: 20-755; Coverage: 100%)
Gene name: BT_3990
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: Z5L, MAN
Ligand Z5L 6 x Z5L
Ligand MAN 6 x MAN
2 bound ligands:
Ligand CA 6 x CA
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P21
Unit cell:
a: 161.26Å b: 68.59Å c: 204.009Å
α: 90° β: 94.66° γ: 90°
R-values:
R R work R free
0.192 0.19 0.244
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The devil lies in the details: how variations in polysaccharide fine-structure impact the physiology and evolution of gut microbes.
Martens et al. (2014)
5 more

5 mentions without citation

Mechanistic insights into a Ca2+-dependent family of alpha-mannosidases in a human gut symbiont.
Zhu et al. (2010)
4 more