› 2x5v

X-ray diffraction
3Å resolution

80 microsecond laue diffraction snapshot from crystals of a photosynthetic reaction centre 3 millisecond following photoactivation.

Released:
DOI: 10.2210/pdb2x5v/pdb
PDB entry 2x5v coloured by chain, front view.
PDB entry 2x5v coloured by chain, side view.
PDB entry 2x5v coloured by chain, top view.
Source organism: Blastochloris viridis
Primary publication:
Light-induced structural changes in a photosynthetic reaction center caught by Laue diffraction.
Wöhri AB, Katona G, Johansson LC, Fritz E, Malmerberg E, Andersson M, Vincent J, Eklund M, Cammarata M, Wulff M, Davidsson J, Groenhof G, Neutze R
Science 328 630-3 (2010)
PMID: 20431017

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-138851 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Photosynthetic reaction center cytochrome c subunit Chain: C
Molecule details ›
Chain: C
Length: 336 amino acids
Theoretical weight: 37.45 KDa
Source organism: Blastochloris viridis
UniProt:
  • Canonical: P07173 (Residues: 21-356; Coverage: 100%)
Gene names: BVIRIDIS_00500, cytC, pufC
Sequence domains: Photosynthetic reaction centre cytochrome C subunit
Structure domains: Photosynthetic Reaction Center, subunit C, domain 2
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 258 amino acids
Theoretical weight: 28.56 KDa
Source organism: Blastochloris viridis
UniProt:
  • Canonical: P06008 (Residues: 1-258; Coverage: 100%)
Gene name: puhA
Sequence domains:
Structure domains:
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 274 amino acids
Theoretical weight: 30.6 KDa
Source organism: Blastochloris viridis
UniProt:
  • Canonical: P06009 (Residues: 1-274; Coverage: 100%)
Gene name: pufL
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 324 amino acids
Theoretical weight: 36.06 KDa
Source organism: Blastochloris viridis
UniProt:
  • Canonical: P06010 (Residues: 1-324; Coverage: 100%)
Gene name: pufM
Sequence domains: Photosynthetic reaction centre protein
Structure domains: Photosystem II protein D1-like

Ligands and Environments

5 bound ligands:
Ligand HEC 4 x HEC
Ligand BCB 4 x BCB
Ligand BPB 2 x BPB
Ligand FE2 1 x FE2
Ligand MQ7 1 x MQ7
1 modified residue:
Ligand FME 1 x FME

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID09
Spacegroup: P21212
Unit cell:
a: 85.693Å b: 143.47Å c: 177.998Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.279 0.277 0.319

Quick links

Citations

13 review citations

Overcoming barriers to membrane protein structure determination.
Bill et al. (2011)
12 more

4 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
3 more