› 2x8c

X-ray diffraction
3.1Å resolution

Thioredoxin glutathione reductase from Schistosoma mansoni with the reduced C-terminal end

Released:
DOI: 10.2210/pdb2x8c/pdb
PDB entry 2x8c coloured by chain, front view.
PDB entry 2x8c coloured by chain, side view.
PDB entry 2x8c coloured by chain, top view.
Source organism: Schistosoma mansoni
Primary publication:
Mapping the catalytic cycle of Schistosoma mansoni thioredoxin glutathione reductase by X-ray crystallography.
Angelucci F, Dimastrogiovanni D, Boumis G, Brunori M, Miele AE, Saccoccia F, Bellelli A
J Biol Chem 285 32557-67 (2010)
PMID: 20659890

Function and Biology Details

Reaction catalysed: 
Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188301 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
thioredoxin-disulfide reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 598 amino acids
Theoretical weight: 65.06 KDa
Source organism: Schistosoma mansoni
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q962Y6 (Residues: 1-598; Coverage: 100%)
Gene name: TGR
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
2 bound ligands:
Ligand PGE 3 x PGE
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 84.492Å b: 87.448Å c: 185.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.216 0.293
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

Schistosomiasis control: praziquantel forever?
Cioli et al. (2014)
6 more

6 mentions without citation

Mapping the catalytic cycle of Schistosoma mansoni thioredoxin glutathione reductase by X-ray crystallography.
Angelucci et al. (2010)
5 more