› 2xbu

X-ray diffraction
1.8Å resolution

Saccharomyces cerevisiae hypoxanthine-guanine phosphoribosyltransferase in complex with GMP (monoclinic crystal form)

Released:
DOI: 10.2210/pdb2xbu/pdb
PDB entry 2xbu coloured by chain, front view.
PDB entry 2xbu coloured by chain, side view.
PDB entry 2xbu coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Functional significance of four successive glycine residues in the pyrophosphate binding loop of fungal 6-oxopurine phosphoribosyltransferases.
Moynié L, Giraud MF, Breton A, Boissier F, Daignan-Fornier B, Dautant A
Protein Sci 21 1185-96 (2012)
PMID: 22610485

Function and Biology Details

Reaction catalysed: 
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170001 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine-guanine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 221 amino acids
Theoretical weight: 25.22 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q04178 (Residues: 1-221; Coverage: 100%)
Gene names: BRA6, D9509.18, HPT1, YDR399W
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand ACT 2 x ACT
Ligand 5GP 2 x 5GP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 51.577Å b: 77.521Å c: 56.655Å
α: 90° β: 95.13° γ: 90°
R-values:
R R work R free
0.169 0.167 0.206
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 citation in other articles

Molecular Recognition of Lipid II by Lantibiotics: Synthesis and Conformational Studies of Analogues of Nisin and Mutacin Rings A and B.
Dickman et al. (2019)
4 more

1 mention without citation

Functional significance of four successive glycine residues in the pyrophosphate binding loop of fungal 6-oxopurine phosphoribosyltransferases.
Moynié et al. (2012)