› 2xhv

X-ray diffraction
1.9Å resolution

HCV-J4 NS5B Polymerase Point Mutant Orthorhombic Crystal Form

Released:
DOI: 10.2210/pdb2xhv/pdb
PDB entry 2xhv coloured by chain, front view.
PDB entry 2xhv coloured by chain, side view.
PDB entry 2xhv coloured by chain, top view.
Source organism: Hepatitis C virus isolate HC-J4
Primary publication:
Further insights into the roles of GTP and the C terminus of the hepatitis C virus polymerase in the initiation of RNA synthesis.
Harrus D, Ahmed-El-Sayed N, Simister PC, Miller S, Triconnet M, Hagedorn CH, Mahias K, Rey FA, Astier-Gin T, Bressanelli S
J Biol Chem 285 32906-32918 (2010)
PMID: 20729191

Function and Biology Details

Reactions catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 579 amino acids
Theoretical weight: 64.64 KDa
Source organism: Hepatitis C virus isolate HC-J4
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O92972 (Residues: 2420-2989; Coverage: 19%)
Sequence domains: Viral RNA dependent RNA polymerase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:
Ligand SO4 18 x SO4
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 105.74Å b: 107.69Å c: 133.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.171 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

The molecular and structural basis of advanced antiviral therapy for hepatitis C virus infection.
Bartenschlager et al. (2013)
11 more

1 mention without citation

Further insights into the roles of GTP and the C terminus of the hepatitis C virus polymerase in the initiation of RNA synthesis.
Harrus et al. (2010)