› 2xj5

X-ray diffraction
1.69Å resolution

The structure of cytochrome c peroxidase Compound II

Released:
DOI: 10.2210/pdb2xj5/pdb
PDB entry 2xj5 coloured by chain, front view.
PDB entry 2xj5 coloured by chain, side view.
PDB entry 2xj5 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Nature of the ferryl heme in compounds I and II.
Gumiero A, Metcalfe CL, Pearson AR, Raven EL, Moody PC
J Biol Chem 286 1260-8 (2011)
PMID: 21062738

Function and Biology Details

Reaction catalysed: 
2 ferrocytochrome c + H(2)O(2) = 2 ferricytochrome c + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132475 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome c peroxidase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 294 amino acids
Theoretical weight: 33.63 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00431 (Residues: 71-361; Coverage: 81%)
Gene names: CCP, CCP1, CPO, YKR066C
Sequence domains: Peroxidase
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
2 bound ligands:
Ligand MPD 6 x MPD
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 51.05Å b: 75.139Å c: 106.919Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.197
Expression system: Escherichia coli BL21(DE3)

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Citations

11 review citations

Oxygen Activation and Radical Transformations in Heme Proteins and Metalloporphyrins.
Huang et al. (2018)
10 more

1 mention without citation

Nature of the ferryl heme in compounds I and II.
Gumiero et al. (2011)