› 2xl9

X-ray diffraction
2.06Å resolution

Structure and metal-loading of a soluble periplasm cupro-protein: Zn- CucA-closed (SeMet)

Released:
DOI: 10.2210/pdb2xl9/pdb
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PDB entry 2xl9 coloured by chain, front view.
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PDB entry 2xl9 coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Structure and metal loading of a soluble periplasm cuproprotein.
Waldron KJ, Firbank SJ, Dainty SJ, Pérez-Rama M, Tottey S, Robinson NJ
J Biol Chem 285 32504-11 (2010)
PMID: 20702411

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159902 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cupin type-1 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 239 amino acids
Theoretical weight: 27.31 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli
UniProt:
  • Canonical: P73600 (Residues: 31-268; Coverage: 89%)
Gene name: sll1785
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand ZN 3 x ZN
Ligand TAM 1 x TAM
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 46.75Å b: 96.97Å c: 114.51Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.19 0.237
Expression system: Escherichia coli

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Citations

6 review citations

Metal preferences and metallation.
Foster et al. (2014)
5 more

1 mention without citation

Structure and metal loading of a soluble periplasm cuproprotein.
Waldron et al. (2010)

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