› 2y0t

X-ray diffraction
1.3Å resolution

The mechanisms of HAMP-mediated signaling in transmembrane receptors - the A291F mutant

Released:
DOI: 10.2210/pdb2y0t/pdb
PDB entry 2y0t coloured by chain, front view.
PDB entry 2y0t coloured by chain, side view.
PDB entry 2y0t coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
The mechanisms of HAMP-mediated signaling in transmembrane receptors.
Ferris HU, Dunin-Horkawicz S, Mondéjar LG, Hulko M, Hantke K, Martin J, Schultz JE, Zeth K, Lupas AN, Coles M
Structure 19 378-85 (2011)
PMID: 21397188

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128065 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HAMP domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 54 amino acids
Theoretical weight: 6.08 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28769 (Residues: 278-331; Coverage: 16%)
Gene name: AF_1503
Sequence domains: HAMP domain
Structure domains: HAMP domain in histidine kinase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 88.07Å b: 34.32Å c: 47.65Å
α: 90° β: 117.79° γ: 90°
R-values:
R R work R free
0.175 0.173 0.209
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Molecular Mechanisms of Two-Component Signal Transduction.
Zschiedrich et al. (2016)
14 more

3 mentions without citation

Fast and anisotropic flexibility-rigidity index for protein flexibility and fluctuation analysis.
Opron et al. (2014)
2 more