› 2yyk

X-ray diffraction
1.6Å resolution

Crystal structure of the mutant of HpaB (T198I, A276G, and R466H)

Released:
DOI: 10.2210/pdb2yyk/pdb
PDB entry 2yyk coloured by chain, front view.
PDB entry 2yyk coloured by chain, side view.
PDB entry 2yyk coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Primary publication:
Crystal structure of the oxygenase component (HpaB) of the 4-hydroxyphenylacetate 3-monooxygenase from Thermus thermophilus HB8.
Kim SH, Hisano T, Takeda K, Iwasaki W, Ebihara A, Miki K
J Biol Chem 282 33107-17 (2007)
PMID: 17804419

Function and Biology Details

Reaction catalysed: 
4-hydroxyphenylacetate + FADH(2) + O(2) = 3,4-dihydroxyphenylacetate + FAD + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178032 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxyphenylacetate 3-monooxygenase oxygenase component Chain: A
Molecule details ›
Chain: A
Length: 481 amino acids
Theoretical weight: 54.35 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SJP8 (Residues: 1-481; Coverage: 100%)
Gene name: TTHA0960
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NA 1 x NA
Ligand ACY 3 x ACY
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: I222
Unit cell:
a: 91.819Å b: 99.612Å c: 131.091Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.195 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Two-Component FAD-Dependent Monooxygenases: Current Knowledge and Biotechnological Opportunities.
Heine et al. (2018)
6 more

1 mention without citation

Crystal Structures of SgcE6 and SgcC, the Two-Component Monooxygenase That Catalyzes Hydroxylation of a Carrier Protein-Tethered Substrate during the Biosynthesis of the Enediyne Antitumor Antibiotic C-1027 in Streptomyces globisporus.
Chang et al. (2016)