› 2yzg

X-ray diffraction
2.3Å resolution

Crystal structure of D-ALA:D-ALA Ligase from Thermus thermophilus HB8

Released:
DOI: 10.2210/pdb2yzg/pdb
PDB entry 2yzg coloured by chain, front view.
PDB entry 2yzg coloured by chain, side view.
PDB entry 2yzg coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Kitamura Y, Yokoyama S, Kuramitsu S, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Reaction catalysed: 
ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177919 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-alanine--D-alanine ligase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 319 amino acids
Theoretical weight: 34.89 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SHZ3 (Residues: 1-319; Coverage: 100%)
Gene names: TTHA1587, ddl
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B2
Spacegroup: C2
Unit cell:
a: 165.224Å b: 56.412Å c: 142.213Å
α: 90° β: 109.08° γ: 90°
R-values:
R R work R free
0.243 0.243 0.29
Expression system: Escherichia coli

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