2z3i

X-ray diffraction
1.8Å resolution

Crystal structure of blasticidin S deaminase (BSD) mutant E56Q complexed with substrate

Released:

Function and Biology Details

Reaction catalysed:
Blasticidin S + H(2)O = deaminohydroxyblasticidin S + NH(3)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
PDBe Complex ID:
PDB-CPX-143003 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Blasticidin-S deaminase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 130 amino acids
Theoretical weight: 13.48 KDa
Source organism: Aspergillus terreus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C2P0 (Residues: 1-130; Coverage: 100%)
Gene name: bsd
Sequence domains: Cytidine and deoxycytidylate deaminase zinc-binding region
Structure domains: Cytidine Deaminase, domain 2

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL45XU
Spacegroup: P212121
Unit cell:
a: 55.33Å b: 69.578Å c: 145.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.22
Expression system: Escherichia coli