› 2z6x

X-ray diffraction
2.3Å resolution

Crystal structure of 22G, the wild-type protein of the photoswitchable GFP-like protein Dronpa

Released:
DOI: 10.2210/pdb2z6x/pdb
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PDB entry 2z6x coloured by chain, front view.
PDB entry 2z6x coloured by chain, side view.
PDB entry 2z6x coloured by chain, top view.
Source organism: Pectiniidae
Primary publication:
Light-dependent regulation of structural flexibility in a photochromic fluorescent protein.
Mizuno H, Mal TK, Wälchli M, Kikuchi A, Fukano T, Ando R, Jeyakanthan J, Taka J, Shiro Y, Ikura M, Miyawaki A
Proc Natl Acad Sci U S A 105 9227-32 (2008)
PMID: 18574155

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178341 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 255 amino acids
Theoretical weight: 29.23 KDa
Source organism: Pectiniidae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5TLG6 (Residues: 1-224; Coverage: 100%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GYC 8 x GYC

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P43
Unit cell:
a: 111.683Å b: 111.683Å c: 156.531Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.278
Expression system: Escherichia coli

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Citations

24 review citations

Genetically encodable fluorescent biosensors for tracking signaling dynamics in living cells.
Newman et al. (2011)
23 more

3 mentions without citation

Light-dependent regulation of structural flexibility in a photochromic fluorescent protein.
Mizuno et al. (2008)
2 more

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