› 2z71

X-ray diffraction
2.6Å resolution

Structure of truncated mutant CYS1GLY of penicillin V acylase from bacillus sphaericus co-crystallized with penicillin V

Released:
DOI: 10.2210/pdb2z71/pdb
PDB entry 2z71 coloured by chain, front view.
PDB entry 2z71 coloured by chain, side view.
PDB entry 2z71 coloured by chain, top view.
Source organism: Lysinibacillus sphaericus
Entry authors: Pathak MC, Brannigan J, Dodson GG, Suresh CG

Function and Biology Details

Reaction catalysed: 
Penicillin + H(2)O = a carboxylate + 6-aminopenicillanate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146100 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillin V acylase Chains: A, C
Molecule details ›
Chains: A, C
Length: 335 amino acids
Theoretical weight: 37.2 KDa
Source organism: Lysinibacillus sphaericus
Expression system: Escherichia coli
UniProt:
  • Canonical: P12256 (Residues: 4-338; Coverage: 99%)
Sequence domains: Linear amide C-N hydrolases, choloylglycine hydrolase family
Structure domains: Penicillin V Acylase; Chain A

Ligands and Environments

1 bound ligand:
Ligand PNV 2 x PNV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2221
Unit cell:
a: 129.73Å b: 158.89Å c: 90.742Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.239 0.27
Expression system: Escherichia coli

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