› 2z8g

X-ray diffraction
1.7Å resolution

Aspergillus niger ATCC9642 isopullulanase complexed with isopanose

Released:
DOI: 10.2210/pdb2z8g/pdb
PDB entry 2z8g coloured by chain, front view.
PDB entry 2z8g coloured by chain, side view.
PDB entry 2z8g coloured by chain, top view.
Source organism: Aspergillus niger
Primary publication:
Crystal structure of Aspergillus niger isopullulanase, a member of glycoside hydrolase family 49.
Mizuno M, Koide A, Yamamura A, Akeboshi H, Yoshida H, Kamitori S, Sakano Y, Nishikawa A, Tonozuka T
J Mol Biol 376 210-20 (2008)
PMID: 18155243

Function and Biology Details

Reaction catalysed: 
Hydrolysis of pullulan to isopanose (6-alpha-maltosylglucose)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126117 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Isopullulanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 549 amino acids
Theoretical weight: 60.04 KDa
Source organism: Aspergillus niger
Expression system: Komagataella pastoris
UniProt:
  • Canonical: O00105 (Residues: 20-564; Coverage: 100%)
Gene name: ipuA
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GLC
Carbohydrate polymer
1 bound ligand:
Ligand NAG 23 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 83.672Å b: 99.387Å c: 134.987Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.211
Expression system: Komagataella pastoris

Quick links

Citations

1 review citation

Characteristics, protein engineering and applications of microbial thermostable pullulanases and pullulan hydrolases.
Nisha et al. (2016)
7 other articles

1 mention without citation

The structure of a family 110 glycoside hydrolase provides insight into the hydrolysis of α-1,3-galactosidic linkages in λ-carrageenan and blood group antigens.
McGuire et al. (2020)