› 2zc0

X-ray diffraction
2.3Å resolution

Crystal structure of an archaeal alanine:glyoxylate aminotransferase

Released:
DOI: 10.2210/pdb2zc0/pdb
PDB entry 2zc0 coloured by chain, front view.
PDB entry 2zc0 coloured by chain, side view.
PDB entry 2zc0 coloured by chain, top view.
Source organism: Thermococcus litoralis
Primary publication:
Structure of an archaeal alanine:glyoxylate aminotransferase.
Sakuraba H, Yoneda K, Takeuchi K, Tsuge H, Katunuma N, Ohshima T
Acta Crystallogr D Biol Crystallogr 64 696-9 (2008)
PMID: 18560158

Function and Biology Details

Reaction catalysed: 
L-alanine + glyoxylate = pyruvate + glycine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189907 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase class I/classII large domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 407 amino acids
Theoretical weight: 45.23 KDa
Source organism: Thermococcus litoralis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9C4M4 (Residues: 1-407; Coverage: 100%)
Gene name: agt
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PMP 4 x PMP
2 bound ligands:
Ligand ZN 4 x ZN
Ligand IMD 4 x IMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P212121
Unit cell:
a: 77.694Å b: 112.519Å c: 207.287Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.236 0.261
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Structure-guided discovery of phenyl-diketo acids as potent inhibitors of M. tuberculosis malate synthase.
Krieger et al. (2012)
1 more