› 2ze0

X-ray diffraction
2Å resolution

Alpha-glucosidase GSJ

Released:
DOI: 10.2210/pdb2ze0/pdb
PDB entry 2ze0 coloured by chain, front view.
PDB entry 2ze0 coloured by chain, side view.
PDB entry 2ze0 coloured by chain, top view.
Source organism: Geobacillus sp. HTA-462
Primary publication:
Crystal structure of GH13 alpha-glucosidase GSJ from one of the deepest sea bacteria.
Shirai T, Hung VS, Morinaka K, Kobayashi T, Ito S
Proteins 73 126-33 (2008)
PMID: 18398906

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-174303 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-amylase Chain: A
Molecule details ›
Chain: A
Length: 555 amino acids
Theoretical weight: 65.26 KDa
Source organism: Geobacillus sp. HTA-462
Expression system: Bacillus subtilis
UniProt:
  • Canonical: Q33E90 (Residues: 1-555; Coverage: 100%)
Gene name: gsj
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: C2
Unit cell:
a: 103.829Å b: 90.621Å c: 72.285Å
α: 90° β: 109.4° γ: 90°
R-values:
R R work R free
0.205 0.202 0.247
Expression system: Bacillus subtilis

Quick links

Citations

5 review citations

α-Amylase: an enzyme specificity found in various families of glycoside hydrolases.
Janeček et al. (2014)
4 more

13 mentions without citation

Advanced Bioinformatics Tools in the Pharmacokinetic Profiles of Natural and Synthetic Compounds with Anti-Diabetic Activity.
Udrea et al. (2021)
12 more