› 2znb

X-ray diffraction
2.15Å resolution

METALLO-BETA-LACTAMASE (CADMIUM-BOUND FORM)

Released:
DOI: 10.2210/pdb2znb/pdb
PDB entry 2znb coloured by chain, front view.
PDB entry 2znb coloured by chain, side view.
PDB entry 2znb coloured by chain, top view.
Source organism: Bacteroides fragilis
Primary publication:
Crystal structures of the cadmium- and mercury-substituted metallo-beta-lactamase from Bacteroides fragilis.
Concha NO, Rasmussen BA, Bush K, Herzberg O
Protein Sci 6 2671-6 (1997)
PMID: 9416622

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150609 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 232 amino acids
Theoretical weight: 25.27 KDa
Source organism: Bacteroides fragilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P25910 (Residues: 18-249; Coverage: 100%)
Gene names: ccrA, cfiA
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

2 bound ligands:
Ligand CD 4 x CD
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P43212
Unit cell:
a: 78.086Å b: 78.086Å c: 139.746Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.182 0.259
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Zinc enzymes.
Coleman JE. (1998)
4 more

1 mention without citation

Evolving carbapenemases: can medicinal chemists advance one step ahead of the coming storm?
Oelschlaeger et al. (2010)