› 2zo6

X-ray diffraction
1.4Å resolution

Crystal Structure of Kusabira-Cyan (KCY), a Cyan-Emitting GFP-Like Protein

Released:
DOI: 10.2210/pdb2zo6/pdb
PDB entry 2zo6 coloured by chain, front view.
PDB entry 2zo6 coloured by chain, side view.
PDB entry 2zo6 coloured by chain, top view.
Source organism: Lithophyllon concinna
Primary publication:
Crystal structure of a new cyan fluorescent protein and its hue-shifted variants.
Kikuchi A, Fukumura E, Karasawa S, Shiro Y, Miyawaki A
Biochemistry 48 5276-83 (2009)
PMID: 19402703

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-163384 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CYAN-EMITTING GFP-LIKE PROTEIN, KUSABIRA-CYAN (KCY) Chain: A
Molecule details ›
Chain: A
Length: 252 amino acids
Theoretical weight: 28.22 KDa
Source organism: Lithophyllon concinna
Expression system: Escherichia coli
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GYS 1 x GYS

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: C2
Unit cell:
a: 94.754Å b: 42.804Å c: 50.699Å
α: 90° β: 96.28° γ: 90°
R-values:
R R work R free
0.164 0.164 0.181
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Blue-Shifted Green Fluorescent Protein Homologues Are Brighter than Enhanced Green Fluorescent Protein under Two-Photon Excitation.
Molina et al. (2017)
1 more

1 mention without citation

Excited state proton transfer in the red fluorescent protein mKeima.
Henderson et al. (2009)