› 2zq7

X-ray diffraction
0.94Å resolution

Apo structure of Class A beta-lactamase Toho-1 E166A/R274N/R276N triple mutant

Released:
DOI: 10.2210/pdb2zq7/pdb
PDB entry 2zq7 coloured by chain, front view.
PDB entry 2zq7 coloured by chain, side view.
PDB entry 2zq7 coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Shimamura T, Nitanai Y, Uchiyama T, Ago H, Matsuzawa H, Miyano M

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175211 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Toho-1 Chain: A
Molecule details ›
Chain: A
Length: 262 amino acids
Theoretical weight: 28.12 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47066 (Residues: 30-291; Coverage: 100%)
Gene name: bla
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: P3221
Unit cell:
a: 72.545Å b: 72.545Å c: 97.656Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.136 0.135 0.147
Expression system: Escherichia coli

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