› 2zts

X-ray diffraction
2.07Å resolution

Crystal structure of KaiC-like protein PH0186 from hyperthermophilic archaea Pyrococcus horikoshii OT3

Released:
DOI: 10.2210/pdb2zts/pdb
PDB entry 2zts coloured by chain, front view.
PDB entry 2zts coloured by chain, side view.
PDB entry 2zts coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
Crystal structure of KaiC-like protein PH0186 from hyperthermophilic archaea Pyrococcus horikoshii OT3.
Kang HJ, Kubota K, Ming H, Miyazono K, Tanokura M
Proteins 75 1035-9 (2009)
PMID: 19274727

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129632 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
KaiC domain-containing protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 251 amino acids
Theoretical weight: 28.88 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O57925 (Residues: 1-251; Coverage: 100%)
Gene name: PH0186
Sequence domains: KaiC
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand ADP 3 x ADP
Ligand MG 3 x MG
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: C2
Unit cell:
a: 173.655Å b: 51.807Å c: 97.468Å
α: 90° β: 122.83° γ: 90°
R-values:
R R work R free
0.199 0.197 0.25
Expression system: Escherichia coli

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Citations

2 review citations

The itty-bitty time machine genetics of the cyanobacterial circadian clock.
Mackey et al. (2011)
1 more

2 mentions without citation

Insights into the binding of Phenyltiocarbamide (PTC) agonist to its target human TAS2R38 bitter receptor.
Biarnés et al. (2010)
1 more