› 2zxa

X-ray diffraction
2.57Å resolution

alpha-L-fucosidase complexed with inhibitor, FNJ-acetyl

Released:
DOI: 10.2210/pdb2zxa/pdb
PDB entry 2zxa coloured by chain, front view.
PDB entry 2zxa coloured by chain, side view.
PDB entry 2zxa coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Structural basis of alpha-fucosidase inhibition by iminocyclitols with K(i) values in the micro- to picomolar range.
Wu HJ, Ho CW, Ko TP, Popat SD, Lin CH, Wang AH
Angew Chem Int Ed Engl 49 337-40 (2010)
PMID: 19967696

Function and Biology Details

Reaction catalysed: 
An alpha-L-fucoside + H(2)O = L-fucose + an alcohol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194507 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-L-fucosidase, putative Chains: A, B
Molecule details ›
Chains: A, B
Length: 455 amino acids
Theoretical weight: 53.1 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WYE2 (Residues: 1-449; Coverage: 100%)
Gene name: TM_0306
Sequence domains: Alpha-L-fucosidase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZXA 2 x ZXA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: R32
Unit cell:
a: 180.562Å b: 180.562Å c: 169.345Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.161 0.225
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The dual role of fucosidases: tool or target.
Jiménez-Pérez et al. (2023)
18 other articles

1 mention without citation

VaProS: a database-integration approach for protein/genome information retrieval.
Gojobori et al. (2016)