› 2zyh

X-ray diffraction
1.83Å resolution

mutant A. Fulgidus lipase S136A complexed with fatty acid fragment

Released:
DOI: 10.2210/pdb2zyh/pdb
PDB entry 2zyh coloured by chain, front view.
PDB entry 2zyh coloured by chain, side view.
PDB entry 2zyh coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Structure of the alkalohyperthermophilic Archaeoglobus fulgidus lipase contains a unique C-terminal domain essential for long-chain substrate binding.
Chen CK, Lee GC, Ko TP, Guo RT, Huang LM, Liu HJ, Ho YF, Shaw JF, Wang AH
J Mol Biol 390 672-85 (2009)
PMID: 19447113

Function and Biology Details

Reaction catalysed: 
Triacylglycerol + H(2)O = diacylglycerol + a carboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128033 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lipase, putative Chains: A, B
Molecule details ›
Chains: A, B
Length: 475 amino acids
Theoretical weight: 52.96 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O28511 (Residues: 1-474; Coverage: 100%)
Gene name: AF_1763
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand R16 2 x R16
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL12B2
Spacegroup: P212121
Unit cell:
a: 91.196Å b: 107.873Å c: 118.896Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.223
Expression system: Escherichia coli

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Citations

7 review citations

The Lid Domain in Lipases: Structural and Functional Determinant of Enzymatic Properties.
Khan et al. (2017)
6 more