› 2zza

X-ray diffraction
2Å resolution

Moritella profunda Dihydrofolate reductase complex with NADP+ and Folate

Released:
DOI: 10.2210/pdb2zza/pdb
PDB entry 2zza coloured by chain, front view.
PDB entry 2zza coloured by chain, side view.
PDB entry 2zza coloured by chain, top view.
Source organism: Moritella profunda
Entry authors: Hata K, Tanaka T, Murakami C, Ohmae E, Gekko K, Shiro Y, Akasaka K

Function and Biology Details

Reaction catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180715 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydrofolate reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 162 amino acids
Theoretical weight: 18.28 KDa
Source organism: Moritella profunda
Expression system: Escherichia coli
UniProt:
  • Canonical: Q70YQ6 (Residues: 1-162; Coverage: 100%)
Gene name: dyrA
Sequence domains: Dihydrofolate reductase
Structure domains: Dihydrofolate Reductase, subunit A

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
2 bound ligands:
Ligand FOL 2 x FOL
Ligand 1PE 1 x 1PE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2221
Unit cell:
a: 56.598Å b: 77.458Å c: 159.819Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.224
Expression system: Escherichia coli

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