› 3a06

X-ray diffraction
2Å resolution

Crystal structure of DXR from Thermooga maritia, in complex with fosmidomycin and NADPH

Released:
DOI: 10.2210/pdb3a06/pdb
PDB entry 3a06 coloured by chain, front view.
PDB entry 3a06 coloured by chain, side view.
PDB entry 3a06 coloured by chain, top view.
Source organism: Thermotoga maritima
Primary publication:
Crystal structure of 1-deoxy-d-xylulose 5-phosphate reductoisomerase from the hyperthermophile Thermotoga maritima for insights into the coordination of conformational changes and an inhibitor binding.
Takenoya M, Ohtaki A, Noguchi K, Endo K, Sasaki Y, Ohsawa K, Yajima S, Yohda M
J Struct Biol 170 532-9 (2010)
PMID: 20353826

Function and Biology Details

Reaction catalysed: 
2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + NADPH
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-deoxy-D-xylulose 5-phosphate reductoisomerase Chains: A, B
Molecule details ›
Chains: A, B
Length: 376 amino acids
Theoretical weight: 42.26 KDa
Source organism: Thermotoga maritima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WZZ1 (Residues: 1-376; Coverage: 100%)
Gene names: TM_0889, dxr
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NDP 3 x NDP
2 bound ligands:
Ligand FOM 2 x FOM
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P43
Unit cell:
a: 108.622Å b: 108.622Å c: 74.662Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.24
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Mechanistic aspects of carotenoid biosynthesis.
Moise et al. (2014)
3 more

7 mentions without citation

Modulation of Dengue/Zika Virus Pathogenicity by Antibody-Dependent Enhancement and Strategies to Protect Against Enhancement in Zika Virus Infection.
Khandia et al. (2018)
6 more