› 3acf

X-ray diffraction
1.6Å resolution

Crystal Structure of Carbohydrate-Binding Module Family 28 from Clostridium josui Cel5A in a ligand-free form

Released:
DOI: 10.2210/pdb3acf/pdb
PDB entry 3acf coloured by chain, front view.
PDB entry 3acf coloured by chain, side view.
PDB entry 3acf coloured by chain, top view.
Source organism: Ruminiclostridium josui
Primary publication:
Recognition of cellooligosaccharides by a family 28 carbohydrate-binding module.
Tsukimoto K, Takada R, Araki Y, Suzuki K, Karita S, Wakagi T, Shoun H, Watanabe T, Fushinobu S
FEBS Lett 584 1205-11 (2010)
PMID: 20159017

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cellulase Chain: A
Molecule details ›
Chain: A
Length: 203 amino acids
Theoretical weight: 22.39 KDa
Source organism: Ruminiclostridium josui
Expression system: Escherichia coli
UniProt:
  • Canonical: Q59290 (Residues: 560-752; Coverage: 21%)
Gene name: celA
Sequence domains: Carbohydrate binding domain (family 17/28)
Structure domains: Galactose-binding domain-like

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 47.164Å b: 63.305Å c: 71.251Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.156 0.191
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Using structure to inform carbohydrate binding module function.
Abbott et al. (2014)
1 more

1 mention without citation

Prediction of carbohydrate binding sites on protein surfaces with 3-dimensional probability density distributions of interacting atoms.
Tsai et al. (2012)