› 3ahw

X-ray diffraction
1.03Å resolution

Crystal Structure of Ustilago sphaerogena Ribonuclease U2 complexed with adenosine 2'-monophosphate

Released:
DOI: 10.2210/pdb3ahw/pdb
PDB entry 3ahw coloured by chain, front view.
PDB entry 3ahw coloured by chain, side view.
PDB entry 3ahw coloured by chain, top view.
Source organism: Ustilago sphaerogena
Primary publication:
Conformational variation revealed by the crystal structure of RNase U2A complexed with Ca ion and 2'-adenylic acid at 1.03 Å resolution.
Noguchi S
Protein Pept Lett 17 1559-61 (2010)
PMID: 20858208

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing adenosine = an (RNA fragment)-3'-adenosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132815 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease U2 Chain: A
Molecule details ›
Chain: A
Length: 114 amino acids
Theoretical weight: 12.39 KDa
Source organism: Ustilago sphaerogena
UniProt:
  • Canonical: P00654 (Residues: 1-114; Coverage: 100%)
Gene name: RNU2
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

2 bound ligands:
Ligand 2AM 1 x 2AM
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P21
Unit cell:
a: 38.994Å b: 35.94Å c: 36.151Å
α: 90° β: 103.42° γ: 90°
R-values:
R R work R free
0.137 0.137 0.144

Quick links

Citations

4 citation in other articles

Protein asparagine deamidation prediction based on structures with machine learning methods.
Jia et al. (2017)
3 more