› 3av7

X-ray diffraction
1.84Å resolution

Crystal structure of Pyrococcus horikoshii kynurenine aminotransferase in complex with PMP, KYN as substrates and KYA as products

Released:
DOI: 10.2210/pdb3av7/pdb
PDB entry 3av7 coloured by chain, front view.
PDB entry 3av7 coloured by chain, side view.
PDB entry 3av7 coloured by chain, top view.
Source organism: Pyrococcus horikoshii OT3
Entry authors: Okada K, Angkawidjaja C, Koga Y, Takano K, Kanaya S

Function and Biology Details

Reaction catalysed: 
(1a) L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129641 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminotransferase class I/classII domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 448 amino acids
Theoretical weight: 51.16 KDa
Source organism: Pyrococcus horikoshii OT3
Expression system: Escherichia coli
UniProt:
  • Canonical: O57946 (Residues: 1-428; Coverage: 100%)
Gene name: PH0207
Sequence domains: Aminotransferase class I and II
Structure domains:

Ligands and Environments


Cofactor: Ligand PMP 2 x PMP
2 bound ligands:
Ligand KYN 2 x KYN
Ligand KYA 2 x KYA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P21
Unit cell:
a: 55.619Å b: 136.529Å c: 55.612Å
α: 90° β: 100.71° γ: 90°
R-values:
R R work R free
0.179 0.176 0.238
Expression system: Escherichia coli

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