› 3ax7

X-ray diffraction
2.34Å resolution

Bovine Xanthine Oxidase, protease cleaved form

Released:
DOI: 10.2210/pdb3ax7/pdb
PDB entry 3ax7 coloured by chain, front view.
PDB entry 3ax7 coloured by chain, side view.
PDB entry 3ax7 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Protein conformational gating of enzymatic activity in xanthine oxidoreductase.
Ishikita H, Eger BT, Okamoto K, Nishino T, Pai EF
J Am Chem Soc 134 999-1009 (2012)
PMID: 22145797

Function and Biology Details

Reactions catalysed: 
Xanthine + NAD(+) + H(2)O = urate + NADH
Xanthine + H(2)O + O(2) = urate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160413 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:

Ligands and Environments


Cofactor: Ligand XAX 2 x XAX

Cofactor: Ligand FAD 2 x FAD
5 bound ligands:
Ligand FES 4 x FES
Ligand SAL 2 x SAL
Ligand BCT 2 x BCT
Ligand GOL 5 x GOL
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 133.477Å b: 73.151Å c: 146.267Å
α: 90° β: 98.18° γ: 90°
R-values:
R R work R free
0.197 0.194 0.24

Quick links

Citations

7 review citations

Proton transfer reactions and hydrogen-bond networks in protein environments.
Ishikita et al. (2014)
6 more

1 mention without citation

In-vitro antigout potential of Alstonia scholaris flower, characterization and prospective ligand-receptor interaction of bioactive lead compound.
Fatima et al. (2023)