› 3axd

X-ray diffraction
1.53Å resolution

The truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase V18Y/W203Y in apo-form

Released:
DOI: 10.2210/pdb3axd/pdb
PDB entry 3axd coloured by chain, front view.
PDB entry 3axd coloured by chain, side view.
PDB entry 3axd coloured by chain, top view.
Source organism: Fibrobacter succinogenes subsp. succinogenes S85
Primary publication:
Rational design to improve thermostability and specific activity of the truncated Fibrobacter succinogenes 1,3-1,4-β-D-glucanase.
Huang JW, Cheng YS, Ko TP, Lin CY, Lai HL, Chen CC, Ma Y, Zheng Y, Huang CH, Zou P, Liu JR, Guo RT
Appl Microbiol Biotechnol 94 111-21 (2012)
PMID: 21959377

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148237 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-glucanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 27.83 KDa
Source organism: Fibrobacter succinogenes subsp. succinogenes S85
Expression system: Escherichia coli
UniProt:
  • Canonical: P17989 (Residues: 25-271; Coverage: 76%)
Gene names: FSU_0226, Fisuc_2961
Sequence domains: Glycosyl hydrolases family 16
Structure domains: Jelly Rolls

Ligands and Environments

2 bound ligands:
Ligand TRS 2 x TRS
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P21
Unit cell:
a: 40.391Å b: 69.963Å c: 81.242Å
α: 90° β: 100.98° γ: 90°
R-values:
R R work R free
0.173 0.173 0.193
Expression system: Escherichia coli

Quick links

Citations

2 review citations

From protein engineering to immobilization: promising strategies for the upgrade of industrial enzymes.
Singh et al. (2013)
1 more