› 3azv

X-ray diffraction
3.1Å resolution

Crystal structure of the receptor binding domain

Released:
DOI: 10.2210/pdb3azv/pdb
PDB entry 3azv coloured by chain, front view.
PDB entry 3azv coloured by chain, side view.
PDB entry 3azv coloured by chain, top view.
Source organism: Clostridium botulinum
Primary publication:
Structural and mutational analyses of the receptor binding domain of botulinum D/C mosaic neurotoxin: insight into the ganglioside binding mechanism.
Nuemket N, Tanaka Y, Tsukamoto K, Tsuji T, Nakamura K, Kozaki S, Yao M, Tanaka I
Biochem Biophys Res Commun 411 433-9 (2011)
PMID: 21749855

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192247 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neurotoxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 443 amino acids
Theoretical weight: 52.23 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9LBR1 (Residues: 857-1285; Coverage: 33%)
Gene name: nt
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 28 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 57.96Å b: 138.57Å c: 162.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.25 0.286
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Botulinum Neurotoxins: Biology, Pharmacology, and Toxicology.
Pirazzini et al. (2017)
2 more