› 3b30

X-ray diffraction
1.97Å resolution

Crystal Structure of F. graminearum TRI101 complexed with Ethyl Coenzyme A

Released:
DOI: 10.2210/pdb3b30/pdb
PDB entry 3b30 coloured by chain, front view.
PDB entry 3b30 coloured by chain, side view.
PDB entry 3b30 coloured by chain, top view.
Source organism: Fusarium graminearum
Primary publication:
Structural and functional characterization of the TRI101 trichothecene 3-O-acetyltransferase from Fusarium sporotrichioides and Fusarium graminearum: kinetic insights to combating Fusarium head blight.
Garvey GS, McCormick SP, Rayment I
J Biol Chem 283 1660-1669 (2008)
PMID: 17923480

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128810 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trichothecene 3-O-acetyltransferase Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 49.45 KDa
Source organism: Fusarium graminearum
Expression system: Escherichia coli
UniProt:
  • Canonical: O42692 (Residues: 1-444; Coverage: 98%)
Gene name: Tri101
Sequence domains: Trichothecene 3-O-acetyltransferase-like N-terminal domain
Structure domains: Chloramphenicol acetyltransferase-like domain

Ligands and Environments

2 bound ligands:
Ligand ETB 1 x ETB
Ligand MPO 1 x MPO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P4212
Unit cell:
a: 123.219Å b: 123.219Å c: 81.308Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.209
Expression system: Escherichia coli

Quick links

Citations

19 review citations

Trichothecenes: from simple to complex mycotoxins.
McCormick et al. (2011)
18 more

3 mentions without citation

Global DNA methylation profiling reveals silencing of a secreted form of Epha7 in mouse and human germinal center B-cell lymphomas.
Dawson et al. (2007)
2 more