› 3b31

X-ray diffraction
2.4Å resolution

Crystal structure of domain III of the Cricket Paralysis Virus IRES RNA

Released:
DOI: 10.2210/pdb3b31/pdb
PDB entry 3b31 coloured by chain, front view.
PDB entry 3b31 coloured by chain, side view.
PDB entry 3b31 coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
tRNA-mRNA mimicry drives translation initiation from a viral IRES.
Costantino DA, Pfingsten JS, Rambo RP, Kieft JS
Nat Struct Mol Biol 15 57-64 (2008)
PMID: 18157151

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196624 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
RNA (29-MER) Chain: A
Molecule details ›
Chain: A
Length: 29 nucleotides
Theoretical weight: 9.33 KDa
Source organism: Synthetic construct
Expression system: Not provided
RNA (5'-R(P*UP*AP*AP*GP*AP*AP*AP*UP*UP*UP*AP*CP*CP*U)-3') Chain: B
Molecule details ›
Chain: B
Length: 14 nucleotides
Theoretical weight: 4.42 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand IRI 3 x IRI
Ligand PO4 1 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU, ALS BEAMLINE 12.3.1
Spacegroup: P41212
Unit cell:
a: 58.724Å b: 58.724Å c: 98.989Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.239 0.269
Expression system: Not provided

Quick links

Citations

42 review citations

Regulation of translation initiation in eukaryotes: mechanisms and biological targets.
Sonenberg et al. (2009)
41 more

6 mentions without citation

Initiation of translation by cricket paralysis virus IRES requires its translocation in the ribosome.
Fernández et al. (2014)
5 more