› 3b3q

X-ray diffraction
2.4Å resolution

Crystal structure of a synaptic adhesion complex

Released:
DOI: 10.2210/pdb3b3q/pdb
PDB entry 3b3q coloured by chain, front view.
PDB entry 3b3q coloured by chain, side view.
PDB entry 3b3q coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for synaptic adhesion mediated by neuroligin-neurexin interactions.
Chen X, Liu H, Shim AH, Focia PJ, He X
Nat Struct Mol Biol 15 50-6 (2008)
PMID: 18084303

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157679 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Neuroligin-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 577 amino acids
Theoretical weight: 64.62 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q99K10 (Residues: 46-635; Coverage: 70%)
Gene names: Kiaa1070, Nlgn1
Sequence domains: Carboxylesterase family
Structure domains: alpha/beta hydrolase
Neurexin-1-beta Chains: E, F
Molecule details ›
Chains: E, F
Length: 197 amino acids
Theoretical weight: 21.46 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P58400 (Residues: 85-296; Coverage: 43%)
  • Best match: P58400-1 (Residues: 85-266)
Gene name: NRXN1
Sequence domains: Laminin G domain
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P212121
Unit cell:
a: 116.59Å b: 125.4Å c: 131.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.242 0.261
Expression system: Spodoptera frugiperda

Quick links

Citations

12 review citations

Neuroligins and neurexins link synaptic function to cognitive disease.
Südhof TC. (2008)
11 more

15 mentions without citation

Neurexins.
Reissner et al. (2013)
14 more