› 3b8s

X-ray diffraction
2Å resolution

Crystal structure of wild-type chitinase A from Vibrio harveyi

Released:
DOI: 10.2210/pdb3b8s/pdb
PDB entry 3b8s coloured by chain, front view.
PDB entry 3b8s coloured by chain, side view.
PDB entry 3b8s coloured by chain, top view.
Source organism: Vibrio harveyi
Primary publication:
Crystal structures of Vibrio harveyi chitinase A complexed with chitooligosaccharides: implications for the catalytic mechanism.
Songsiriritthigul C, Pantoom S, Aguda AH, Robinson RC, Suginta W
J Struct Biol 162 491-9 (2008)
PMID: 18467126

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189592 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GH18 domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 584 amino acids
Theoretical weight: 63.84 KDa
Source organism: Vibrio harveyi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9AMP1 (Residues: 22-597; Coverage: 70%)
Gene name: chiA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P1
Unit cell:
a: 60.267Å b: 64.283Å c: 83.522Å
α: 91.74° β: 91.18° γ: 112.91°
R-values:
R R work R free
0.17 0.168 0.205
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The type II secretion system: biogenesis, molecular architecture and mechanism.
Korotkov et al. (2012)
5 more

4 mentions without citation

Crystal structures of Bacillus cereus NCTU2 chitinase complexes with chitooligomers reveal novel substrate binding for catalysis: a chitinase without chitin binding and insertion domains.
Hsieh et al. (2010)
3 more