› 3bai

X-ray diffraction
1.9Å resolution

Human Pancreatic Alpha Amylase with Bound Nitrate

Released:
DOI: 10.2210/pdb3bai/pdb
PDB entry 3bai coloured by chain, front view.
PDB entry 3bai coloured by chain, side view.
PDB entry 3bai coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Alternative catalytic anions differentially modulate human alpha-amylase activity and specificity.
Maurus R, Begum A, Williams LK, Fredriksen JR, Zhang R, Withers SG, Brayer GD
Biochemistry 47 3332-44 (2008)
PMID: 18284212

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138163 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pancreatic alpha-amylase Chain: A
Molecule details ›
Chain: A
Length: 496 amino acids
Theoretical weight: 55.93 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P04746 (Residues: 16-511; Coverage: 100%)
Gene name: AMY2A
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NAG 1 x NAG
Ligand NO3 1 x NO3
Ligand CA 1 x CA
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 52.99Å b: 69.11Å c: 132.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.169 0.208
Expression system: Komagataella pastoris

Quick links

Citations

6 review citations

Determinants of salivary alpha-amylase in humans and methodological considerations.
Rohleder et al. (2009)
5 more

4 mentions without citation

Alpha-Glucosidase and Alpha-Amylase Inhibitory Activities, Molecular Docking, and Antioxidant Capacities of Salvia aurita Constituents.
Etsassala et al. (2020)
3 more