› 3bfd

X-ray diffraction
2Å resolution

Crystal Structure of the Class A beta-lactamase SED-G238C mutant from Citrobacter sedlakii

Released:
DOI: 10.2210/pdb3bfd/pdb
PDB entry 3bfd coloured by chain, front view.
PDB entry 3bfd coloured by chain, side view.
PDB entry 3bfd coloured by chain, top view.
Source organism: Citrobacter sedlakii
Entry authors: Pernot L, Petrella S, Sougakoff W

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188115 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 263 amino acids
Theoretical weight: 28.45 KDa
Source organism: Citrobacter sedlakii
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q93PQ0 (Residues: 33-295; Coverage: 99%)
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

1 bound ligand:
Ligand CAC 4 x CAC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2
Unit cell:
a: 187.642Å b: 73.2Å c: 103.893Å
α: 90° β: 121.89° γ: 90°
R-values:
R R work R free
0.188 0.183 0.214
Expression system: Escherichia coli BL21

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