› 3bff

X-ray diffraction
1.9Å resolution

class A beta-lactamase SED-G238C complexed with faropenem

Released:
DOI: 10.2210/pdb3bff/pdb
PDB entry 3bff coloured by chain, front view.
PDB entry 3bff coloured by chain, side view.
PDB entry 3bff coloured by chain, top view.
Source organism: Citrobacter sedlakii
Entry authors: Pernot L, Petrella S, Sougakoff W

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188115 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 262 amino acids
Theoretical weight: 28.33 KDa
Source organism: Citrobacter sedlakii
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q93PQ0 (Residues: 34-295; Coverage: 98%)
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

3 bound ligands:
Ligand SCN 6 x SCN
Ligand SFR 4 x SFR
Ligand FPM 1 x FPM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2
Unit cell:
a: 187.116Å b: 73.244Å c: 103.504Å
α: 90° β: 121.69° γ: 90°
R-values:
R R work R free
0.189 0.18 0.205
Expression system: Escherichia coli BL21

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