› 3bm5

X-ray diffraction
2.4Å resolution

Crystal structure of O-acetyl-serine sulfhydrylase from Entamoeba histolytica in complex with cysteine

Released:
DOI: 10.2210/pdb3bm5/pdb
PDB entry 3bm5 coloured by chain, front view.
PDB entry 3bm5 coloured by chain, side view.
PDB entry 3bm5 coloured by chain, top view.
Source organism: Entamoeba histolytica HM-1:IMSS
Primary publication:
Crystal structure of native O-acetyl-serine sulfhydrylase from Entamoeba histolytica and its complex with cysteine: structural evidence for cysteine binding and lack of interactions with serine acetyl transferase.
Chinthalapudi K, Kumar M, Kumar S, Jain S, Alam N, Gourinath S
Proteins 72 1222-32 (2008)
PMID: 18350570

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127538 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan synthase beta chain-like PALP domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 338 amino acids
Theoretical weight: 36.92 KDa
Source organism: Entamoeba histolytica HM-1:IMSS
Expression system: Escherichia coli
UniProt:
  • Canonical: O15570 (Residues: 2-337; Coverage: 100%)
Sequence domains: Pyridoxal-phosphate dependent enzyme
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand SO4 6 x SO4
Ligand PLP 2 x PLP
Ligand CYS 1 x CYS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P41
Unit cell:
a: 80.36Å b: 80.36Å c: 111.761Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.231
Expression system: Escherichia coli

Quick links

Citations

1 review citation

The cysteine regulatory complex from plants and microbes: what was old is new again.
Jez et al. (2013)
17 other articles

2 mentions without citation

Insights into multifaceted activities of CysK for therapeutic interventions.
Joshi et al. (2019)
1 more