› 3brf

X-ray diffraction
2.47Å resolution

CSL (Lag-1) bound to DNA with Lin-12 RAM peptide, C2221

Released:
DOI: 10.2210/pdb3brf/pdb
PDB entry 3brf coloured by chain, front view.
PDB entry 3brf coloured by chain, side view.
PDB entry 3brf coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
RAM-induced allostery facilitates assembly of a notch pathway active transcription complex.
Friedmann DR, Wilson JJ, Kovall RA
J Biol Chem 283 14781-91 (2008)
PMID: 18381292

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-116103 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Lin-12 and glp-1 phenotype protein 1, isoform a Chain: A
Molecule details ›
Chain: A
Length: 478 amino acids
Theoretical weight: 53.85 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
Structure domains:
Protein lin-12 Chain: D
Molecule details ›
Chain: D
Length: 14 amino acids
Theoretical weight: 1.65 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli
UniProt:
  • Canonical: P14585 (Residues: 938-950; Coverage: 1%)
Gene names: R107.8, lin-12
DNA (5'-D(*DTP*DTP*DAP*DCP*DTP*DGP*DTP*DGP*DGP*DGP*DAP*DAP*DAP*DGP*DA)-3') Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.67 KDa
DNA (5'-D(*DAP*DAP*DTP*DCP*DTP*DTP*DTP*DCP*DCP*DCP*DAP*DCP*DAP*DGP*DT)-3') Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.5 KDa

Ligands and Environments

1 bound ligand:
Ligand SOR 2 x SOR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2221
Unit cell:
a: 62.944Å b: 95.972Å c: 223.63Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.23 0.277
Expression system: Escherichia coli

Quick links

Citations

10 review citations

The canonical Notch signaling pathway: unfolding the activation mechanism.
Kopan et al. (2009)
9 more

6 mentions without citation

The molecular logic of Notch signaling--a structural and biochemical perspective.
Gordon et al. (2008)
5 more