› 3bs7

X-ray diffraction
1.9Å resolution

Crystal structure of the Sterile Alpha Motif (SAM) domain of Hyphen/Aveugle

Released:
DOI: 10.2210/pdb3bs7/pdb
PDB entry 3bs7 coloured by chain, front view.
PDB entry 3bs7 coloured by chain, side view.
PDB entry 3bs7 coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling.
Rajakulendran T, Sahmi M, Kurinov I, Tyers M, Therrien M, Sicheri F
Proc Natl Acad Sci U S A 105 2836-41 (2008)
PMID: 18287031

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185315 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein aveugle Chains: A, B
Molecule details ›
Chains: A, B
Length: 78 amino acids
Theoretical weight: 9.49 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8ML92 (Residues: 21-98; Coverage: 74%)
Gene names: CG30476, ave
Sequence domains: SAM domain (Sterile alpha motif)
Structure domains: Transcription Factor, Ets-1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2221
Unit cell:
a: 56.005Å b: 88.47Å c: 73.279Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.214 0.265
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Grading the commercial optical biosensor literature-Class of 2008: 'The Mighty Binders'.
Rich et al. (2010)
4 more

1 mention without citation

CNK and HYP form a discrete dimer by their SAM domains to mediate RAF kinase signaling.
Rajakulendran et al. (2008)