› 3bw3

X-ray diffraction
2.2Å resolution

Crystal structures and site-directed mutagenesis study of nitroalkane oxidase from Streptomyces ansochromogenes

Released:
DOI: 10.2210/pdb3bw3/pdb
Model geometry
Fit model/data
PDB entry 3bw3 coloured by chain, front view.
PDB entry 3bw3 coloured by chain, side view.
PDB entry 3bw3 coloured by chain, top view.
Source organism: Streptomyces ansochromogenes
Primary publication:
Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes.
Li Y, Gao Z, Hou H, Li L, Zhang J, Yang H, Dong Y, Tan H
Biochem Biophys Res Commun 405 344-8 (2011)
PMID: 21147069

Function and Biology Details

Reaction catalysed: 
A nitroalkane + H(2)O + O(2) = an aldehyde or ketone + nitrite + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190297 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Propionate 3-nitronate monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 369 amino acids
Theoretical weight: 38.52 KDa
Source organism: Streptomyces ansochromogenes
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9FDD4 (Residues: 1-363; Coverage: 100%)
Gene name: 2-npdl
Sequence domains: Nitronate monooxygenase
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
2 bound ligands:
Ligand NIE 1 x NIE
Ligand MPD 2 x MPD
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BSRF BEAMLINE 1W2B
Spacegroup: I4122
Unit cell:
a: 113.465Å b: 113.465Å c: 115.585Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.207
Expression system: Escherichia coli

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Citations

3 review citations

Flavoprotein oxidases: classification and applications.
Dijkman et al. (2013)
2 more