› 3bxf

X-ray diffraction
1.7Å resolution

Crystal structure of effector binding domain of central glycolytic gene regulator (CggR) from Bacillus subtilis in complex with effector fructose-1,6-bisphosphate

Released:
DOI: 10.2210/pdb3bxf/pdb
PDB entry 3bxf coloured by chain, front view.
PDB entry 3bxf coloured by chain, side view.
PDB entry 3bxf coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structures of the effector-binding domain of repressor Central glycolytic gene Regulator from Bacillus subtilis reveal ligand-induced structural changes upon binding of several glycolytic intermediates.
Rezácová P, Kozísek M, Moy SF, Sieglová I, Joachimiak A, Machius M, Otwinowski Z
Mol Microbiol 69 895-910 (2008)
PMID: 18554327

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128441 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Central glycolytic genes regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 27.56 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O32253 (Residues: 89-340; Coverage: 74%)
Gene names: BSU33950, cggR, yvbQ
Sequence domains: Putative sugar-binding domain
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand FBP 1 x FBP
Ligand CL 4 x CL
Ligand 13P 1 x 13P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 51.447Å b: 83.245Å c: 116.457Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.201 0.257
Expression system: Escherichia coli

Quick links

Citations

3 review citations

The emerging role of native mass spectrometry in characterizing the structure and dynamics of macromolecular complexes.
Boeri Erba et al. (2015)
2 more

3 mentions without citation

Crystal structures of the effector-binding domain of repressor Central glycolytic gene Regulator from Bacillus subtilis reveal ligand-induced structural changes upon binding of several glycolytic intermediates.
Rezácová et al. (2008)
2 more