› 3c15

X-ray diffraction
2.78Å resolution

Complex of GS-Alpha with the Catalytic Domains of Mammalian Adenylyl Cyclase: Complex with Pyrophosphate and Mg

Released:
DOI: 10.2210/pdb3c15/pdb
PDB entry 3c15 coloured by chain, front view.
PDB entry 3c15 coloured by chain, side view.
PDB entry 3c15 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for inhibition of mammalian adenylyl cyclase by calcium.
Mou TC, Masada N, Cooper DM, Sprang SR
Biochemistry 48 3387-97 (2009)
PMID: 19243146

Function and Biology Details

Reaction catalysed: 
ATP = 3',5'-cyclic AMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-138237 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Adenylate cyclase type 5 Chain: A
Molecule details ›
Chain: A
Length: 225 amino acids
Theoretical weight: 25.53 KDa
Source organism: Canis lupus familiaris
Expression system: Escherichia coli
UniProt:
  • Canonical: P30803 (Residues: 444-661; Coverage: 17%)
Gene name: ADCY5
Sequence domains: Adenylate and Guanylate cyclase catalytic domain
Structure domains: Nucleotide cyclase
Adenylate cyclase type 2 Chain: B
Molecule details ›
Chain: B
Length: 212 amino acids
Theoretical weight: 23.72 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P26769 (Residues: 870-1081; Coverage: 19%)
Gene name: Adcy2
Sequence domains: Adenylate and Guanylate cyclase catalytic domain
Structure domains: Nucleotide cyclase
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: C
Molecule details ›
Chain: C
Length: 402 amino acids
Theoretical weight: 46.71 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04896 (Residues: 1-394; Coverage: 100%)
Gene names: GNAS, GNAS1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MG 1 x MG
Ligand FOK 1 x FOK
Ligand POP 1 x POP
Ligand CL 1 x CL
Ligand GSP 1 x GSP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 117.51Å b: 133.08Å c: 70.108Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.237 0.291
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Regulation by Ca2+-signaling pathways of adenylyl cyclases.
Halls et al. (2011)
8 more

4 mentions without citation

International Union of Basic and Clinical Pharmacology. CI. Structures and Small Molecule Modulators of Mammalian Adenylyl Cyclases.
Dessauer et al. (2017)
3 more