› 3c4u

X-ray diffraction
1.83Å resolution

Structure of class II fructose-biphosphate aldolase from helicobacter pylori

Released:
DOI: 10.2210/pdb3c4u/pdb
PDB entry 3c4u coloured by chain, front view.
PDB entry 3c4u coloured by chain, side view.
PDB entry 3c4u coloured by chain, top view.
Source organism: Helicobacter pylori
Primary publication:
Synthesis and biochemical evaluation of selective inhibitors of class II fructose bisphosphate aldolases: towards new synthetic antibiotics.
Fonvielle M, Coinçon M, Daher R, Desbenoit N, Kosieradzka K, Barilone N, Gicquel B, Sygusch J, Jackson M, Therisod M
Chemistry 14 8521-9 (2008)
PMID: 18688832

Function and Biology Details

Reaction catalysed: 
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157394 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-bisphosphate aldolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 33.8 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli
UniProt:
  • Canonical: P56109 (Residues: 1-307; Coverage: 100%)
Gene names: HP_0176, fba
Sequence domains: Fructose-bisphosphate aldolase class-II
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand NA 2 x NA
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 39.023Å b: 82.56Å c: 91.272Å
α: 90° β: 99.66° γ: 90°
R-values:
R R work R free
0.188 0.186 0.207
Expression system: Escherichia coli

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Citations

1 review citation

Targeting Metalloenzymes for Therapeutic Intervention.
Chen et al. (2019)
16 other articles

4 mentions without citation

Active site loop dynamics of a class IIa fructose 1,6-bisphosphate aldolase from Mycobacterium tuberculosis.
Pegan et al. (2013)
3 more