› 3c52

X-ray diffraction
2.3Å resolution

Class II fructose-1,6-bisphosphate aldolase from helicobacter pylori in complex with phosphoglycolohydroxamic acid, a competitive inhibitor

Released:
DOI: 10.2210/pdb3c52/pdb
PDB entry 3c52 coloured by chain, front view.
PDB entry 3c52 coloured by chain, side view.
PDB entry 3c52 coloured by chain, top view.
Source organism: Helicobacter pylori
Primary publication:
Synthesis and biochemical evaluation of selective inhibitors of class II fructose bisphosphate aldolases: towards new synthetic antibiotics.
Fonvielle M, Coinçon M, Daher R, Desbenoit N, Kosieradzka K, Barilone N, Gicquel B, Sygusch J, Jackson M, Therisod M
Chemistry 14 8521-9 (2008)
PMID: 18688832

Function and Biology Details

Reaction catalysed: 
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157394 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fructose-bisphosphate aldolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 307 amino acids
Theoretical weight: 33.8 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli
UniProt:
  • Canonical: P56109 (Residues: 1-307; Coverage: 100%)
Gene names: HP_0176, fba
Sequence domains: Fructose-bisphosphate aldolase class-II
Structure domains: Aldolase class I

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 2 x CA
Ligand NA 2 x NA
Ligand PGH 2 x PGH
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 39.242Å b: 85.305Å c: 90.818Å
α: 90° β: 100.16° γ: 90°
R-values:
R R work R free
0.204 0.204 0.249
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Targeting Metalloenzymes for Therapeutic Intervention.
Chen et al. (2019)
17 other articles

4 mentions without citation

Specificity and detection of insulin-reactive CD4+ T cells in type 1 diabetes in the nonobese diabetic (NOD) mouse.
Crawford et al. (2011)
3 more