› 3c76

X-ray diffraction
1.07Å resolution

1.07 A crystal structure of L133V mutant of nitrophorin 4 from Rhodnius prolixus complexed with ammonia at PH 7.5

Released:
DOI: 10.2210/pdb3c76/pdb
PDB entry 3c76 coloured by chain, front view.
PDB entry 3c76 coloured by chain, side view.
PDB entry 3c76 coloured by chain, top view.
Source organism: Rhodnius prolixus
Entry authors: Amoia AM, Montfort WR

Function and Biology Details

Reaction catalysed: 
3 nitrite + 2 H(+) = 2 nitric oxide + nitrate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitrophorin-4 Chain: X
Molecule details ›
Chain: X
Length: 184 amino acids
Theoretical weight: 20.28 KDa
Source organism: Rhodnius prolixus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q94734 (Residues: 22-205; Coverage: 100%)
Sequence domains: Nitrophorin
Structure domains: Lipocalin

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
1 bound ligand:
Ligand NH3 1 x NH3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: C2
Unit cell:
a: 70.32Å b: 42.847Å c: 52.871Å
α: 90° β: 94.26° γ: 90°
R-values:
R R work R free
0.141 0.139 0.165
Expression system: Escherichia coli BL21(DE3)

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