› 3ctl

X-ray diffraction
2.2Å resolution

Crystal structure of D-Allulose 6-Phosphate 3-Epimerase from Escherichia coli K12 complexed with D-glucitol 6-phosphate and magnesium

Released:
DOI: 10.2210/pdb3ctl/pdb
PDB entry 3ctl coloured by chain, front view.
PDB entry 3ctl coloured by chain, side view.
PDB entry 3ctl coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis for substrate specificity in phosphate binding (beta/alpha)8-barrels: D-allulose 6-phosphate 3-epimerase from Escherichia coli K-12.
Chan KK, Fedorov AA, Fedorov EV, Almo SC, Gerlt JA
Biochemistry 47 9608-17 (2008)
PMID: 18700786

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo hexamer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152430 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-allulose-6-phosphate 3-epimerase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 231 amino acids
Theoretical weight: 26.14 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P32719 (Residues: 1-231; Coverage: 100%)
Gene names: JW4046, alsE, b4085, yjcU
Sequence domains: Ribulose-phosphate 3 epimerase family
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand S6P 6 x S6P
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 75.706Å b: 129.04Å c: 154.874Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.243 0.265
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Specificity in transition state binding: the Pauling model revisited.
Amyes et al. (2013)
2 more

1 mention without citation

Structural basis for substrate specificity in phosphate binding (beta/alpha)8-barrels: D-allulose 6-phosphate 3-epimerase from Escherichia coli K-12.
Chan et al. (2008)