› 3d67

X-ray diffraction
3.4Å resolution

Crystal structure of Thrombin-Activatable Fibrinolysis Inhibitor (TAFI) in complex with 2-guanidino-ethyl-mercaptosuccinic acid (GEMSA)

Released:
DOI: 10.2210/pdb3d67/pdb
PDB entry 3d67 coloured by chain, front view.
PDB entry 3d67 coloured by chain, side view.
PDB entry 3d67 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structures of TAFI elucidate the inactivation mechanism of activated TAFI: a novel mechanism for enzyme autoregulation.
Marx PF, Brondijk TH, Plug T, Romijn RA, Hemrika W, Meijers JC, Huizinga EG
Blood 112 2803-9 (2008)
PMID: 18559974

Function and Biology Details

Reaction catalysed: 
Release of C-terminal Arg and Lys from a polypeptide.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188550 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxypeptidase B2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 424 amino acids
Theoretical weight: 48.67 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q96IY4 (Residues: 24-423; Coverage: 100%)
Gene name: CPB2
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand NAG 11 x NAG
Ligand ZN 3 x ZN
Ligand GEM 3 x GEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P3121
Unit cell:
a: 161.05Å b: 161.05Å c: 138.99Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.198 0.252
Expression system: Homo sapiens

Quick links

Citations

12 review citations

New insights into the molecular mechanisms of the fibrinolytic system.
Rijken et al. (2009)
11 more

2 mentions without citation

Thrombin Activatable Fibrinolysis Inhibitor (TAFI): An Updated Narrative Review.
Sillen et al. (2021)
1 more