› 3d8v

X-ray diffraction
2.55Å resolution

Crystal structure of GlmU from Mycobacterium tuberculosis in complex with uridine-diphosphate-N-acetylglucosamine

Released:
DOI: 10.2210/pdb3d8v/pdb
PDB entry 3d8v coloured by chain, front view.
PDB entry 3d8v coloured by chain, side view.
PDB entry 3d8v coloured by chain, top view.
Source organism: Mycobacterium tuberculosis
Primary publication:
Structure and function of GlmU from Mycobacterium tuberculosis.
Zhang Z, Bulloch EM, Bunker RD, Baker EN, Squire CJ
Acta Crystallogr D Biol Crystallogr 65 275-83 (2009)
PMID: 19237750

Function and Biology Details

Reactions catalysed: 
Acetyl-CoA + alpha-D-glucosamine 1-phosphate = CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate = diphosphate + UDP-N-acetyl-alpha-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161813 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional protein GlmU Chain: A
Molecule details ›
Chain: A
Length: 495 amino acids
Theoretical weight: 51.64 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WMN3 (Residues: 1-495; Coverage: 100%)
Gene names: Rv1018c, glmU
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand UD1 1 x UD1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: R32
Unit cell:
a: 114.238Å b: 114.238Å c: 361.796Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.199 0.244
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Mycobacterial cell wall biosynthesis: a multifaceted antibiotic target.
Abrahams et al. (2018)
5 more

5 mentions without citation

The structural biology of enzymes involved in natural product glycosylation.
Singh et al. (2012)
4 more